Clostridium difficile (C.diff.) Laboratory Test Collection,Storage,Transport

CLOSTRIDIUM DIFFICILE TOXIN, PCR
Label Name: C DIFF
Lab Discipline: Microbiology
Subdiscipline: Bacteriology
Feces (Stool)
Container & Volume
Age Group Container Volume
0  – 18 Years Sterile Specimen Cup 5  ML
Label Reminders
Must include patient name, MRN, date/time of collection and collector’s initials.

 

 

Collection Notes
All:
  • ACCEPTABLE SPECIMEN: Feces

    Collect specimen as follows:
    1. Collect feces in a clean, dry container or bedpan not contaminated with urine, residual soap, or disinfectants.
    2. Transfer appropriate volume of feces to a clean dry tightly capped specimen cup.
    3. Submit double-bagged specimen cup immediately to laboratory.

  • NOTES:
    1. Patients should be passing at least 3 unformed or watery stool specimens in a 24-hour period. Most patients have more than 3 episodes of watery, foul-smelling stools per day.
    2. Soft specimen is defined as specimen assuming the shape of its container (unformed).
    3. Formed or hard fecal specimens will not be tested.
    4. Repeat testing following a negative test during the same episode of diarrhea is NOT recommended for at least 7-days because of high sensitivity (between 98-99% and with a 99% negative predictive value).
Storage
REFRIGERATE IF TRANSPORT IS DELAYED. Specimens can be stored at 2-8 degrees C for 24 hours before significant degredation of the toxin is noted. SUBMIT WITH COLD PACKS OR ON ICE IF TRANSPORT WILL TAKE > 1 HOUR.
Transport
DELIVER IMMEDIATELY TO MICROBIOLOGY IN A TIGHTLY SEALED CONTAINER WITH NO EXTERNAL SPILLAGE.
REFRIGERATE IF TRANSPORT IS DELAYED. SUBMIT WITH COLD PACKS OR ON ICE IF TRANSPORT WILL TAKE >1 HOUR.
Causes for Rejection
All:
  • 1. Specimen not labeled with patient’s name, MRN #, date/time of collection, collector’s initials.
    2. Container leaking.
    3. Specimen received > 2 hours after collection unless submitted on ice or cold pack or with note that specimen stored in refrigerator prior to transport.
    4. Formed or hard specimen.
    5. Specimen in Cary-Blair transport, Para-Pak (formalin/PVA) or in diaper.
    6. Specimen on swab.
    7. Patient has negative test within last 7 days.
    8. Patient has positive test within last 14 days.
Turn Around Time –  Routine: 1 day