Tag Archives: C difficile studies

Clostridium difficile Research: Bacteriophage Combinations Significantly Reduce C. diff. Growth

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Bacteriophage Combinations Significantly Reduce Clostridium difficile Growth In Vitro and Proliferation In Vivo

 

 

The microbiome dysbiosis caused by antibiotic treatment has been associated with both susceptibility to and relapse of Clostridium difficile infection (CDI). Bacteriophage (phage) therapy offers target specificity and dose amplification in situ, but few studies have focused on its use in CDI treatment. This mainly reflects the lack of strictly virulent phages that target this pathogen. While it is widely accepted that temperate phages are unsuitable for therapeutic purposes due to their transduction potential, analysis of seven C. difficile phages confirmed that this impact could be curtailed by the application of multiple phage types. Here, host range analysis of six myoviruses and one siphovirus was conducted on 80 strains representing 21 major epidemic and clinically severe ribotypes. The phages had complementary coverage, lysing 18 and 62 of the ribotypes and strains tested, respectively. Single-phage treatments of ribotype 076, 014/020, and 027 strains showed an initial reduction in the bacterial load followed by the emergence of phage-resistant colonies. However, these colonies remained susceptible to infection with an unrelated phage. In contrast, specific phage combinations caused the complete lysis of C. difficile in vitro and prevented the appearance of resistant/lysogenic clones. Using a hamster model, the oral delivery of optimized phage combinations resulted in reduced C. difficile colonization at 36 h postinfection. Interestingly, free phages were recovered from the bowel at this time. In a challenge model of the disease, phage treatment delayed the onset of symptoms by 33 h compared to the time of onset of symptoms in untreated animals. These data demonstrate the therapeutic potential of phage combinations to treat CDI.

 

To read the article in its entirety please click on the following link:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4750681/

 

Article shared by Dr. Martha Clokie, Ph.D., Member of the C Diff Foundation
Research and Development Committee

Clostridium difficile Infection (CDI, C. diff. ) for Healthcare Providers

Patients admitted to an ICU for Clostridium difficile infection were at risk for developing subsequent C. difficile infections, according to recent research.

To read this article in its entirety:

http://www.healio.com/infectious-disease/gastrointestinal-infections/news/online/%7Be22ec9f0-c474-4753-9b95-2084d4f9b177%7D/icu-patients-admitted-with-c-difficile-colonization-at-risk-for-subsequent-infections

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Patients with Clostridium difficile infection (CDI) warranting admission to the ICU may benefit from a treatment regimen of combined oral vancomycin and IV metronidazole, according to recent findings.

To read article in its entirety: 

http://www.healio.com/infectious-disease/gastrointestinal-infections/news/online/%7B86429037-44a1-4ac3-9241-b2a8f31d9312%7D/c-difficile-patients-benefit-from-oral-vancomycin-iv-metronidazole-combination

In a retrospective, observational, comparative study, researchers evaluated 88 critically ill adult patients with C. difficile who were admitted to the ICU at Wake Forest Baptist Medical Center between June 2007 and September 2012. All patients were treated for CDI with oral vancomycin, and those in the combination therapy group received concomitant metronidazole intravenously for a minimum of 72 hours. Patients were matched and equally placed within either the combination or vancomycin-only groups using the Acute Physiology and Chronic Health Evaluation II (APACHE II) metric. The patients were clinically and demographically comparable, although the combination therapy group had a higher prevalence of moderate-to-severe renal disease.

The study’s primary outcome was in-hospital death, and secondary outcomes included clinical success at days 6, 10 and 21; hospital length of stay after diagnosis of CDI; and duration of ICU stay after diagnosis of CDI. Multivariable analysis was used to identify factors independently correlated with survival.